Tutorial: The Basic Setup

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When starting Mr. Kick for the first time settings will be more or less like the ones found in the configuration file ttDefault.mat. We'll use these settings as the basis and mainly discuss changes to this setup.

Simple Stimulus-Response

The first example should resemble the following: At some regular interval we'll stimulate and measure the response, i.e. sample a sequence of data (a sweep). The stimulation may be electrical, magnetic or any other that can be trigged by a digital pulse.

This could be used for measuring the Hoffmann reflex, which will be used as an example: A nerve is stimulated electrically and the response is measured in the EMG of the enervated muscle.

The settings below are available from the file ttBasic0.mat.

Acquisition

The direct M-response is seen around 15 ms after stimulation. The H-reflex follows around 40 ms after stimulation (m. Soleus). A sweep length of 100 ms is sufficient. It may be a good idea to include a pre-trig period of say 20 ms to monitor muscle activity prior to the stimulation.

In order to measure a correct peak-peak value of the M and H-responses a relatively high sample rate must be applied, e.g. 10 kHz.

Analog Input

For this purpose we'll need only one channel: The EMG from one muscle. Label that channel, e.g. "Soleus" (clear On/Sampled for all other). The sample rate should be set to High to achieve the 10 kHz. No filtering should be applied as this may affect the peak-peak measurement of the M- and H-responses.

Make sure the Gain or Sensitivity setting reflects the setting of the EMG amplifier in use. A sensitivity of 500 µV/V might be a reasonable starting point. The signal of this channel is grouped as EMG, and the SI-unit is Volt (V). The EMG amplifier in this example supplies voltages in the range from -10 to +10 V.

Classification: Y-Analysis

Classification will not be in use. But we can still monitor the H-reflex during data acquisition: Let's display the peak-peak amplitude of the signal in the time interval from 32 to 52 ms after stimulation. This is set up in the Y-Analysis section of the Classification settings (lower left).

You can show/hide the Y-analysis display via the Windows menu. It will automatically hide as no classification is applied.

Trigger

Trigger settings control the sampling of sweeps, i.e. what will start (trigger) the acquisition. We want to sample stimulus-responses at a regular interval, say every four seconds. We'll choose Internal as the trig Mode/Source for Mr. Kick to control this. In order to avoid anticipation it may be a good idea not to stimulate exactly every four seconds. By setting Refractory to 3.5 s and Max Interval to 4.5 s the inter stimuli interval will vary randomly ±0.5 s around a 4 s mean.

Event Timing

For this simple example we simply connect the trigger input of the stimulator to the output of event timer #0. In the hardware manual coming with the MIO-board in use this output is labeled GPCTR0_OUT.

When trigger mode is Internal (or digital external) the output pulse from event timer #0 is always synchronized to time zero in every sweep.

Only the Duration and Pulse Polarity settings are relevant here: The Duration controls the duration of the pulse and may be used to control the duration of the stimulus if the stimulator supports this. Pulse Polarity should be set to High if the stimulator is trigged by a low-to-high pulse transition or at high level.

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